phenotype microarray mammalian plates pm-m1 Search Results


90
Biolog Inc phenotype microarray mammalian plates pm-m1
Phenotype Microarray Mammalian Plates Pm M1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phenotype microarray mammalian plates pm-m1/product/Biolog Inc
Average 90 stars, based on 1 article reviews
phenotype microarray mammalian plates pm-m1 - by Bioz Stars, 2026-04
90/100 stars
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Biolog Inc if-m1 media
A ) Flt3L bone marrow derived DCs <t>(FLDCs)</t> generated from mice were exposed to 4-hour PFA-killed spores on Biolog M1 Carbon Source Plates with metabolic activity measured with addition of redox dye. B ) Graph <t>displays</t> <t>FLDC</t> redox signal in culture with PFA-killed spores when incubated on each compound, which is normalised to the percentage of activity detected when cultured in α-D-Glucose. Compounds which caused significant metabolic activity of FLDCs compared to negative control are coloured in red. Schematic shows the points in which identified nutrients can feed into glycolysis. C ) FLDCs generated from mice were precultured in basal media prior to addition of spores in the presence of specified nutrients for 6h. D ) Representative flow cytometry overlays display the gMFI of co- stimulatory molecules (CD40 and CD86), CCR7 and MHCII on FL-cDC2s c after exposure to spores whilst individually supplemented with glucose, fructose, dextrin, inosine, glycerol-3-phosphate, mannose or galactose. Graphs display the fold change difference in expression compared to FL-cDC2s not exposed to spores in basal media. B) data from two independent experiments ( n = 5 FLDCs derived from biologically independent animals). D ) data from two independent experiments ( n = 7 FLDCs derived from biologically independent animals). Statistical significance determined by B ) Kruskal Wallis ANOVA with Dunn’s multiple-comparison post-hoc test vs negative control or D ) one-way ANOVA with tukey’s multiple comparison test between indicated groups. *P < 0.05, **P<0.01, ***P < 0.001, **** P<0.0001.
If M1 Media, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/if-m1 media/product/Biolog Inc
Average 90 stars, based on 1 article reviews
if-m1 media - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

90
Biolog Inc phenotype microarray-mammalian 2 biolog plates
A ) Flt3L bone marrow derived DCs <t>(FLDCs)</t> generated from mice were exposed to 4-hour PFA-killed spores on Biolog M1 Carbon Source Plates with metabolic activity measured with addition of redox dye. B ) Graph <t>displays</t> <t>FLDC</t> redox signal in culture with PFA-killed spores when incubated on each compound, which is normalised to the percentage of activity detected when cultured in α-D-Glucose. Compounds which caused significant metabolic activity of FLDCs compared to negative control are coloured in red. Schematic shows the points in which identified nutrients can feed into glycolysis. C ) FLDCs generated from mice were precultured in basal media prior to addition of spores in the presence of specified nutrients for 6h. D ) Representative flow cytometry overlays display the gMFI of co- stimulatory molecules (CD40 and CD86), CCR7 and MHCII on FL-cDC2s c after exposure to spores whilst individually supplemented with glucose, fructose, dextrin, inosine, glycerol-3-phosphate, mannose or galactose. Graphs display the fold change difference in expression compared to FL-cDC2s not exposed to spores in basal media. B) data from two independent experiments ( n = 5 FLDCs derived from biologically independent animals). D ) data from two independent experiments ( n = 7 FLDCs derived from biologically independent animals). Statistical significance determined by B ) Kruskal Wallis ANOVA with Dunn’s multiple-comparison post-hoc test vs negative control or D ) one-way ANOVA with tukey’s multiple comparison test between indicated groups. *P < 0.05, **P<0.01, ***P < 0.001, **** P<0.0001.
Phenotype Microarray Mammalian 2 Biolog Plates, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phenotype microarray-mammalian 2 biolog plates/product/Biolog Inc
Average 90 stars, based on 1 article reviews
phenotype microarray-mammalian 2 biolog plates - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

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A ) Flt3L bone marrow derived DCs (FLDCs) generated from mice were exposed to 4-hour PFA-killed spores on Biolog M1 Carbon Source Plates with metabolic activity measured with addition of redox dye. B ) Graph displays FLDC redox signal in culture with PFA-killed spores when incubated on each compound, which is normalised to the percentage of activity detected when cultured in α-D-Glucose. Compounds which caused significant metabolic activity of FLDCs compared to negative control are coloured in red. Schematic shows the points in which identified nutrients can feed into glycolysis. C ) FLDCs generated from mice were precultured in basal media prior to addition of spores in the presence of specified nutrients for 6h. D ) Representative flow cytometry overlays display the gMFI of co- stimulatory molecules (CD40 and CD86), CCR7 and MHCII on FL-cDC2s c after exposure to spores whilst individually supplemented with glucose, fructose, dextrin, inosine, glycerol-3-phosphate, mannose or galactose. Graphs display the fold change difference in expression compared to FL-cDC2s not exposed to spores in basal media. B) data from two independent experiments ( n = 5 FLDCs derived from biologically independent animals). D ) data from two independent experiments ( n = 7 FLDCs derived from biologically independent animals). Statistical significance determined by B ) Kruskal Wallis ANOVA with Dunn’s multiple-comparison post-hoc test vs negative control or D ) one-way ANOVA with tukey’s multiple comparison test between indicated groups. *P < 0.05, **P<0.01, ***P < 0.001, **** P<0.0001.

Journal: bioRxiv

Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose

doi: 10.1101/2025.07.13.664342

Figure Lengend Snippet: A ) Flt3L bone marrow derived DCs (FLDCs) generated from mice were exposed to 4-hour PFA-killed spores on Biolog M1 Carbon Source Plates with metabolic activity measured with addition of redox dye. B ) Graph displays FLDC redox signal in culture with PFA-killed spores when incubated on each compound, which is normalised to the percentage of activity detected when cultured in α-D-Glucose. Compounds which caused significant metabolic activity of FLDCs compared to negative control are coloured in red. Schematic shows the points in which identified nutrients can feed into glycolysis. C ) FLDCs generated from mice were precultured in basal media prior to addition of spores in the presence of specified nutrients for 6h. D ) Representative flow cytometry overlays display the gMFI of co- stimulatory molecules (CD40 and CD86), CCR7 and MHCII on FL-cDC2s c after exposure to spores whilst individually supplemented with glucose, fructose, dextrin, inosine, glycerol-3-phosphate, mannose or galactose. Graphs display the fold change difference in expression compared to FL-cDC2s not exposed to spores in basal media. B) data from two independent experiments ( n = 5 FLDCs derived from biologically independent animals). D ) data from two independent experiments ( n = 7 FLDCs derived from biologically independent animals). Statistical significance determined by B ) Kruskal Wallis ANOVA with Dunn’s multiple-comparison post-hoc test vs negative control or D ) one-way ANOVA with tukey’s multiple comparison test between indicated groups. *P < 0.05, **P<0.01, ***P < 0.001, **** P<0.0001.

Article Snippet: In FLDC omnilog experiments, cultured FLDCs were washed twice in IF-M1 (IF-M1, Biolog, 5% dialysed FCS, 0.2 mM L-Glutamine) media followed by plating on a Phenotype MicroArray Mammalian plate PM-M1 (Biolog) at 1 × 10 5 cells/well in IF-M1 media.

Techniques: Derivative Assay, Generated, Activity Assay, Incubation, Cell Culture, Negative Control, Flow Cytometry, Expressing, Comparison